| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | A.G. | Club/Country | Time |
| 2 | 1 | Northampton SC - A | Northampton | 4:10.00 | |
| 3 | 3 | Derventio Excel - A | Derventio | 4:04.00 | |
| 4 | 5 | Nova Centurion SC | Nova Cent'n | 3:57.70 | |
| 5 | 4 | Loughborough Uni | Loughboro Un | 4:00.00 | |
| 6 | 2 | City of Leicester SC | Co Leicester | 4:09.12 | |