| Final | |||||
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 0 | 1 | Jessica Mitchell | 16 | CarltonForum | 29.20 |
| 1 | 2 | Evie Richards | 16 | Co Leicester | 28.80 |
| 2 | 3 | Bethany Eagle-Brown | 16 | Deepings | 28.63 |
| 3 | 4 | Olivia Humphrey | 16 | Co Derby | 28.35 |
| 4 | 5 | Betsy Wizard | 16 | Northampton | 27.40 |
| 5 | 6 | Jennifer Horn | 16 | Lincoln TSA | 27.49 |
| 6 | 7 | Mia Slevin | 16 | Derventio | 28.36 |
| 7 | 8 | Bethany Walker | 16 | Melton M'bry | 28.75 |
| 8 | 9 | Chloe Quinn | 16 | Nova Cent'n | 28.97 |
| 9 | 10 | Isabella Askham | 16 | Long Eaton | 29.32 |