| Final | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 1 | Milen Merritt | 12 | Melton M'bry | 41.45 |
| 2 | 2 | Amelia Sheil | 12 | Derby Excel | 41.27 |
| 3 | 3 | Sophie Lane | 12 | Co Leicester | 40.91 |
| 4 | 4 | Lily-May Clark | 11 | Melton M'bry | 40.28 |
| 5 | 5 | Connie Kyle | 12 | Co Leicester | 40.65 |
| 6 | 6 | Emily Edwards | 12 | Repton | 41.24 |
| 7 | 7 | Sara Vasilevska | 12 | Nova Cent'n | 41.42 |
| 8 | 8 | Keira Parker | 12 | Derby Excel | 41.62 |