| Final | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 1 | Amelie Ratliff | 13 | Derby Excel | 34.99 |
| 2 | 2 | Maya Almohammed | 13 | NottmLeander | 34.72 |
| 3 | 3 | Evelyn Connor | 13 | Ripley | 34.20 |
| 4 | 4 | Emma Jane Rantzen | 13 | BelperMarlin | 32.45 |
| 5 | 5 | Tallulah-Mae Rautenbac | 13 | Co Leicester | 33.53 |
| 6 | 6 | Chloe Gent | 13 | Coalville | 34.65 |
| 7 | 7 | Daisie Taylor | 13 | Worksop | 34.77 |
| 8 | 8 | Emilie Webb | 13 | Grantham | 35.08 |