| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 1 | Francesca Allmark-Palm | 37 | Derbyshire | : . |
| 2 | 2 | Samantha Caffery | 39 | Staffs | : . |
| 3 | 3 | Victoria Ellis-Vowles | 41 | Notts | : . |
| 4 | 4 | Clare Wilkinson | 49 | Warwicks | : . |
| 5 | 5 | Laura Cranshaw | 38 | Worcs | : . |
| 6 | 6 | Sarah Brooksbank | 48 | Leics | : . |
| 7 | 7 | Amy Vaughan | 39 | Northants | : . |
| 8 | 8 | Julia Slater | 44 | Shropshire | : . |
| 9 | 9 | Rachel Day | 40 | Lincolnshire | : . |