| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 1 | Maddie Haywood | 25 | Derbyshire | : . |
| 2 | 2 | Athena Heavey | 26 | Staffs | : . |
| 3 | 3 | Olivia Handley | 29 | Notts | : . |
| 4 | 4 | Amie Hammonds | 28 | Warwicks | : . |
| 5 | 5 | Megan Holder | 25 | Worcs | : . |
| 6 | 6 | Jessica Sutton | 28 | Leics | : . |
| 7 | 7 | Rose Higginson-Burford | 29 | Northants | : . |
| 8 | 8 | Nicola Edwards | 42 | Shropshire | : . |